ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (12): 2251-2257.doi: 10.11843/j.issn.0366-6964.2015.12.017

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Isolation and Identification of Arcanobacterium pyogenes from Goats and Pathological Observation of Naturally Infected Case

ZHANG Su-hui1,2,FU Li-zhi1,2* ,ZHU Mai-xun1,XU Guo-yang1,XU Deng-feng1   

  1. (1.Chongqing Academy of Animal Sciences,Chongqing 402460,China;2.Chongqing Engineering Research Center for Goat,Chongqing 402460,China)
  • Received:2015-04-17 Online:2015-12-23 Published:2015-12-23

Abstract:

This study aimed to confirm the pathogens from purulent lymph goats infected naturally by Arcanobacterium pyogenes. The pathogens were isolated and cultured,identified by pathological method and 16S rRNA analysis.The result of bacterium analysis showed that the pathogen was Arcanobacterium pyogenes,the credibility was 88% identified by VITEK2 Compact automated microbial analysis system and homology was 99% by 16S rRNA identification.The strain was highly sensitive to β-lactams antibiotics and fluoroquinolones,but resistant to sulfa drugs.Pathological sections showed the sick goat appeared myocardial fibrosis,lung and renal tubular lumen with serous-fibrinous degeneration,inflammatory cell infiltration induced hepatitis,spleen was serous-purulent inflammation and front lymph nodes near shoulders appeared steatosis.Thus we can summary that the pathogen of the goat lymphadenitis was Arcanobacterium pyogenes.Histopathological changes were the main organs suppurative inflammation.

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